| Types | DnaRegion
|
| Roles | engineered_region
Intermediate
|
| Sequences | BBa_K933002_sequence (Version 1)
|
Description
This sequence has the available space to insert repeating codon sequences in an effort to determine codon optimization in E.coli. It is prefaced by a weak RBS, a short leader sequence, and contains restriction enzyme sites that allow for the insertion of the repeat sequences. Two reporters-mCherry and GFP-show the degradation of tRNA as the repeated sequences interfere with natural translation. mCherry should change with changing sequences, and GFP should remain constant.
Notes
An alternative RBS site was used to reduce mCherry expression in this construct.
Source
This part was partially assembled by a Penn State Ph.D student in Dr. Thomas Richard's lab named Mike Speer. Completion of the codon optimization project was performed by iGEM members. An alternative RBS site was used to reduce mCherry expression.
