BBa_K933004

BBa_K933004 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K933004

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Chris Cetnar

Date created: 2012-10-10 11:00:00

Date modified: 2015-05-08 01:13:47

Palindromic sequence #1 in directionality analysis construct

Details

• Types: DnaRegion
• Roles: engineered_region; Device

Description

This part is a palindromic sequence designed to be bidirectional. The specific part has the directionality construct with palindrome #1 (BD#1) as the inserted promoter. By digesting at the restriction site xba1 and Spe1 this will remove the promoter sequence, one can then ligate in different promoters at the restriction sites to measure the directionality of different promoter sequences.

Notes

Oligos were designed with sticky end restriction sites xba1 & spe1

Source

Oligos annealed

Other Properties

• igem#sampleStatus: No part sequence
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K933004/1

Member of these Collections

• iGEM Parts Registry