BBa_K936014

BBa_K936014 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K936014

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Nicholas Csicsery

Date created: 2012-08-08 11:00:00

Date modified: 2015-05-08 01:13:47

pelB-LC-Cutinase with polyhistidine-tag

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K936014_sequence (Version 1)

Description

pelB leader sequence attached to enzyme LC-Cutinase protein coding sequence in order to allow the cell to bring the enzyme to the periplasm for easier secretion. The polyhistidine-tag allows for us to purify the produced enzyme.

Notes

We designed the pelB tag to be immediately in front of the LC-cutinase gene.

Source

This part was created from other BioBrick parts.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
pelB	1,66	feature/tag, tag
LC-Cutinase	67,945	feature/protein, CDS
Polyhistidine-tag	946,963	tag, feature/tag

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K936014/1

Member of these Collections

• iGEM Parts Registry