BBa_K942006

BBa_K942006 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K942006

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Luis Mario Leal Garza

Date created: 2012-09-25 11:00:00

Date modified: 2015-05-08 01:13:48

scFvIgE+GFP

Details

• Types: DnaRegion
• Roles: Reporter; engineered_region
• Sequences: BBa_K942006_sequence (Version 1)

Description

This part is a fusion protein made from a murine scFv specific to human IgE at the N-terminal and a yeast-enhanced green fluorescent protein (yEGFP) at the C-terminal part of the protein.

About safety
The part itself does not present any biohazard characteristic, but it is important to remember that it is designed to work in Pichia pastoris and that a biosecurity chamber is recommended to handle this organism.

Notes

The fusion between scFv IgE and GFP is achieved by a (GGGGS)4 linker.

Source

This part is a fusion protein made from a murine scFv specific to human IgE.

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K942006/1

Member of these Collections

• iGEM Parts Registry