BBa_K944001

BBa_K944001 Version 1

Component

This part has been discontinued.

Source:
http://parts.igem.org/Part:BBa_K944001
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Natasha G??mez
Date created: 2012-09-24 11:00:00
Date modified: 2015-05-08 01:13:48

Transcriptional Regulator for Cyanide Inducible Promoter



Types
DnaRegion

Roles
promoter

Regulatory

Sequences BBa_K944001_sequence (Version 1)

Description

The cynF gene codes for a ?sigma 54-dependent transcriptional regulator of 648-amino-acid residues which is not present in other bacterial cyn gene clusters., which belongs to the FIS (factor for inversion stimulation) family of regulatory proteins. P. pseudoalcaligenes CynF shared 59% identity with R. palustris CynF (YP_485671) and 58% identity with Bradyrhizobium japonicum CynF (NP_772375). This Transcriptional Regulator, cynF, works in conjuction with the putative promoter
positively regulated by cyanate, cyanide, and some cyanometallic complexes and negatively regulated by ammonium.

Notes

This part had 3 illegal Pst1 restriction sites.

Source

This part came from the genomic sequence of Pseudomonas pseudoalcaligenes CECT5344.

igem#experience
None
 
igem#sampleStatus
Discontinued
igem#status
Deleted
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K944001/1