Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Natasha G??mez
Date created: 2012-09-25 11:00:00
Date modified: 2015-05-08 01:13:48
Transcriptional Regulator for Cyanide Inducible Promoter
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K944002_sequence (Version 1) |
Description
The cynF gene codes for a sigma ?54-dependent transcriptional regulator of 648-amino-acid residues, which belongs to the FIS (factor for inversion stimulation) family of regulatory proteins. P. pseudoalcaligenes CynF shared 59% identity with R. palustris CynF (YP_485671) and 58% identity with Bradyrhizobium japonicum CynF (NP_772375). This Transcriptional regulator works along with the cyanide and cyanide compounds inducible promoter in the natural ocurring cyanase operon from P. pseudoalcaligenes.Notes
This part had 3 illegal Pst1 restriction sites.Source
This part came from the genomic sequence of Pseudomonas pseudoalcaligenes CECT5344 and was synthesized by IDT technologies.| Sequence Annotation | Location | Component / Role(s) |
| cynF Transcriptional Regulator start codon silent mutation silent mutation silent mutation stop codon | 1,1932 1,1932 1,3 890,890 1253,1253 1277,1277 1930,1932 | sequence_feature feature/dna feature/BioBrick engineered_region feature/start start_codon feature/s_mutation point_mutation point_mutation feature/s_mutation point_mutation feature/s_mutation stop_codon feature/stop |