BBa_K950004

BBa_K950004 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K950004
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jakob Matthes
Date created: 2012-07-28 11:00:00
Date modified: 2015-05-08 01:13:49

firefly luciferase



    • Details

    Types
    DnaRegion

    Roles
    engineered_region

    Reporter

    Sequences BBa_K950004_sequence (Version 1)

    Description

    This is a optimized firefly luciferase (C-terminal modification, see design notes).

    Notes

    Primers were designed to replace the C-terminal SKL aminoacids with KL.

    Source

    The original source is the Firefly luciferase, see http://www.ncbi.nlm.nih.gov/nuccore/AB644228.1

    The modification is covered by 'One-step measurement of firefly luciferase activity in yeast' (http://www.ncbi.nlm.nih.gov/pubmed/14558144).

    Sequence Annotation Location Component / Role(s)
    luciferase
    XbaI
    		1,1650
    48,54
    		feature/cds CDS
    feature/misc sequence_feature

    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#topLevel
    BBa_K950004/1
     

    Attachments

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