Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Daniel Winter
Date created: 2012-09-22 11:00:00
Date modified: 2015-05-08 01:13:49
Deinococcus radiodurans Bacteriophytochrome with RBS (E. coli Codon Optimised)
| Types | DnaRegion |
| Roles | engineered_region Translational_Unit |
| Sequences | BBa_K953001_sequence (Version 1) |
Description
Deinococcus radiodurans bacteriophytochrome, codon optimised for expression in E. coli. In the presence of biliverdin the bacteriophytochrome will undergo a conformation change. This complex can then absorb red light(620-750 nm) to excite the complex and result in a phenotypic change from blue to green. This can be reversed by far-red light (700-800 nm) or will revert from green to blue over time.As E. coli does not produce biliverdin, heme oxygenase (Part BBa_K953000) can be coupled with this part to activate the oxidation of heme to produce biliverdin or by the addition of biliverdin to the cells.
Notes
Optimised for expression in E. coliSource
Deinococcus radiodurans| Sequence Annotation | Location | Component / Role(s) |
| RBS BBa_K953001 Start Codon Bacteriophytochrome Stop Codon | 1,22 1,2296 23,25 26,2290 2291,2296 | feature/rbs ribosome_entry_site engineered_region feature/BioBrick start_codon feature/start feature/cds CDS stop_codon feature/stop |