BBa_K953004

BBa_K953004 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K953004
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: David Dubow
Date created: 2012-09-24 11:00:00
Date modified: 2015-05-08 01:13:49

Hemeoxygenase with RBS (E. coli Codon Optimised)



    • Details

    Types
    DnaRegion

    Roles
    engineered_region

    Translational_Unit

    Sequences BBa_K953004_sequence (Version 1)

    Description

    E. coli codon optimised Synechocystis Heme Oxygenase with a Ribosome Binding Site (RBS), oxidises Heme into biliverdin which absorbs light in the 360 nm and 650 nm wavelength producing a green colouration.

    Notes

    Codon optimisation for E. coli, whilst staying within the GC constraints of gibson assembly.

    Source

    Gibson assembly

    Sequence Annotation Location Component / Role(s)
    RBS
    Start Codon
    Heme Oxygenase
    Stop Codon
    		1,21
    23,25
    26,742
    743,748
    		ribosome_entry_site feature/rbs
    feature/start start_codon
    CDS feature/cds
    stop_codon feature/stop

    igem#experience
    None
     
    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K953004/1
     

    Attachments

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