Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Dylan Patrick Webster
Date created: 2012-07-26 11:00:00
Date modified: 2015-05-08 01:13:49
Arsenic inducible mtrB
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K957001_sequence (Version 1) |
Description
The purpose of this part is to upregulate mtrB expression in response to arsenic. When expressed in a Shewanella strain lacking mtrB on the chromosome, this composite part will function as a component of a biosensor. Specifically, when such a complemented strain is inoculated in a microbial electrochemical system, current output will increase in response to arsenic.Within the genetic circuit, arsR acts as a negative autoregulator, repressing not only the expression of downstream mtrB, but also its own expression. Upon association with arsenic salts, ArsR dissociates from the operator of the arsenic inducible promoter, upregulating expression of downstream protein. Because mtrB activity is requisite for functionality of the mtr electron pathway in Shewanella spp., arsenic thus induces electron shuttling through the mtr pathway.
Notes
AscI cutsite included downstream of arsR and upstream of mtrB to facilitate inducible promoter switching.Source
Arsenic-sensitive component of this composite part was originally derived from the arsenic detoxification operon of E. coli strain JM109; BioBrick part BBa_J33201 was the source of DNA.mtrB is originally derived from the mtrCAB operon of Shewanella oneidensis MR-1; BioBrick part BBa_K098994 was used as DNA source.
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J33201 ArsR binding site -35 sequence -10 sequence rbs arsR mtrB BBa_K098989 BBa_B0034 BBa_K098994 BBa_B0012 T7 TE polya BBa_B0011 stem_loop AscI cut site | 1,472 36,54 57,62 80,85 108,112 119,472 508,2601 508,2601 490,501 490,2704 2610,2650 2617,2636 2637,2650 2659,2704 2671,2693 474,481 | engineered_region feature/BioBrick operator feature/operator feature/promoter promoter feature/promoter promoter feature/rbs ribosome_entry_site feature/cds CDS feature/cds CDS feature/BioBrick engineered_region feature/BioBrick engineered_region feature/BioBrick engineered_region feature/BioBrick engineered_region feature/stem_loop stem_loop polyA_site feature/polya engineered_region feature/BioBrick stem_loop feature/stem_loop sequence_feature feature/misc |