Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Steven Tiwen Chen & Dylan Patrick Webster
Date created: 2012-10-27 11:00:00
Date modified: 2015-05-08 01:13:49
Salicylate inducible MtrB-polyHis, alternate
| Types | DnaRegion |
| Roles | Other sequence_feature |
| Sequences | BBa_K957013_sequence (Version 1) |
Description
This part includes the mtrB coding sequence with a poly-His tag appended to the C-terminus.The purpose of this part is to upregulate mtrB expression in response to salicylate. When expressed in a Shewanella strain lacking mtrB on the chromosome, this composite part will function as a component of a biosensor. Specifically, when such a complemented strain is inoculated in a microbial electrochemical system, current output will increase in response to salicylate. If coupled to the expression of a BioBrick part that degrades naphthalene to salicylate, this part will function as part of a naphthalene biosensor.
Because of the His tag, this part may also be used for in vitro characterization of mtrB, and will also facilitate the purification of mtrB to obtain its crystal structure.
Notes
Constructed using non-BioBrick AscI cutsite of BBa_K957000.Source
mtrB is originally derived from the mtrCAB operon of Shewanella oneidensis MR-1; BioBrick part BBa_K098994 was used as DNA source.The salicylate sensitive region was isolated via PCR from a plasmid containing BBa_K228227, designed by PKU_Beijing 2009.