BBa_K957014

BBa_K957014 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K957014
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Steven Tiwen Chen & Dylan Patrick Webster
Date created: 2012-10-27 11:00:00
Date modified: 2015-05-08 01:13:49

Salicylate inducible polHis-MtrB, alternate



Types
DnaRegion

Roles
sequence_feature

Other

Sequences BBa_K957014_sequence (Version 1)

Description

This part includes the mtrB coding sequence with a poly-His tag appended to the N-terminus.

The purpose of this part is to upregulate mtrB expression in response to salicylate. When expressed in a Shewanella strain lacking mtrB on the chromosome, this composite part will function as a component of a biosensor. Specifically, when such a complemented strain is inoculated in a microbial electrochemical system, current output will increase in response to salicylate. If coupled to the expression of a BioBrick part that degrades naphthalene to salicylate, this part will function as part of a naphthalene biosensor.

Because of the His tag, this part may also be used for in vitro characterization of mtrB, and will also facilitate the purification of mtrB to obtain its crystal structure.

Notes

Constructed using non-BioBrick AscI cutsite of BBa_K957000.

Source

mtrB is originally derived from the mtrCAB operon of Shewanella oneidensis MR-1; BioBrick part BBa_K098994 was used as DNA source.

The salicylate sensitive region was isolated via PCR from a plasmid containing BBa_K228227, designed by PKU_Beijing 2009.

igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K957014/1