Types | DnaRegion
|
Roles | Coding
CDS
|
Sequences | BBa_M10011_sequence (Version 1)
|
Description
Circularly permutated OmpG with a backbone opening in loop 6 of the native protein.
Circular permutation re-orders the protein so that both termini are exposed to the extra-cellular environment.
CPG_L6 is an outer membrane carrier protein that can be used to display other proteins on the outer membrane. "Display proteins" can be fused to the N-termini of {
Notes
Needed to design appropriate linkers (to fuse the N and C termini of the native protien). Used 6aa linker (CDS: GGATCTAAGTCTCGTCGC) from PMID: 18480093.
The opening was created in loop 2 (aa 59-60) because the residues of the mature protein are presumably disordered because they are not visible in electron density maps.
Source
E. coli MG1655 genomic DNA