BBa_M10011

BBa_M10011 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_M10011
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jennifer Brophy
Date created: 2009-04-30 11:00:00
Date modified: 2015-05-08 01:13:51


CPG_L6!}



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_M10011_sequence (Version 1)

Description

Circularly permutated OmpG with a backbone opening in loop 6 of the native protein.

Circular permutation re-orders the protein so that both termini are exposed to the extra-cellular environment.

CPG_L6 is an outer membrane carrier protein that can be used to display other proteins on the outer membrane. "Display proteins" can be fused to the N-termini of {

Notes

Needed to design appropriate linkers (to fuse the N and C termini of the native protien). Used 6aa linker (CDS: GGATCTAAGTCTCGTCGC) from PMID: 18480093.

The opening was created in loop 2 (aa 59-60) because the residues of the mature protein are presumably disordered because they are not visible in electron density maps.

Source

E. coli MG1655 genomic DNA

Sequence Annotation Location Component / Role(s)
Linker
Native N terminus
Native C terminus
172,189
190,862
1,171
feature/misc sequence_feature
feature/protein CDS
feature/protein CDS
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_M10011/1