BBa_M10020

BBa_M10020 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_M10020
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nikhil Sharma
Date created: 2009-05-09 11:00:00
Date modified: 2015-05-08 01:13:51


TraT˃
} C-terminal Display Protein



Types
DnaRegion

Roles
Other

sequence_feature

Sequences BBa_M10020_sequence (Version 1)

Description

later

Notes

-Had to remove the prepro sequence

Source

Construction of {} part

PCR Ons001F/Ons002R on TraT (700 bp, BamHI/EcoRI)
Digest pBca9495CA-Bca1144#5 (BamHI/EcoRI, 3039+910, L)
Product is pBca9495CA-M10020 {}


Ons001F Forward oligo for TraT
ccataGAATTCatgAGATCTtgtggtgcgatgagcacagc
Ons002R Reverse oligo for TraT
ctgatGGATCCgagaatatttgcgattgatttgg

igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_M10020/1