Types | DnaRegion
|
Roles | Other
sequence_feature
|
Sequences | BBa_M10020_sequence (Version 1)
|
Description
later
Notes
-Had to remove the prepro sequence
Source
Construction of {} part
PCR Ons001F/Ons002R on TraT (700 bp, BamHI/EcoRI)
Digest pBca9495CA-Bca1144#5 (BamHI/EcoRI, 3039+910, L)
Product is pBca9495CA-M10020 {}
Ons001F Forward oligo for TraT
ccataGAATTCatgAGATCTtgtggtgcgatgagcacagc
Ons002R Reverse oligo for TraT
ctgatGGATCCgagaatatttgcgattgatttgg