BBa_M10021

BBa_M10021 Version 1

Component

Source: http://parts.igem.org/Part:BBa_M10021

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Nikhil Sharma

Date created: 2009-05-09 11:00:00

Date modified: 2015-05-08 01:13:51

{˂
Poly_bind˃
} passenger protein

Details

• Types: DnaRegion
• Roles: sequence_feature; Other
• Sequences: BBa_M10021_sequence (Version 1)

Description

later

Notes

-Removed prepro sequences

Source

Made from PCR assembly from synthetic sources.

Construction of Polystyrene-Binding Peptide

Wobble Ons003F/Ons004R (67bp, EcoRI/BamHI)
Sub into pBca9495AK-Bca1144#5 (EcoRI/BamHI, 3171+910, L)
Product is pBca9495AK-M10021 {}
----
Ons003F Forward construction of Poly_bind basic part ccataGAATTCatgAGATCTttcaaattctggctatacgaacatg
Ons004R Reverse construction of Poly_bind basic part ctgatGGATCCcccccgtattacatgttcgtatagccagaat

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_M10021/1

Member of these Collections

• iGEM Parts Registry