Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sadao Ota
Date created: 2009-05-09 11:00:00
Date modified: 2015-05-08 01:13:51
{˂
Ag43_long!} for Ag 43 autotransporter display system, including passenger domain
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_M10035_sequence (Version 1) |
Description
This part is in BglBricks Standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at:BglBricks Standard Description Page
This part consists of an actual transporter portion and also passenger domain of an antigen 43 autotransporter display system based on this reference: PMID: 12107137.
Notes
The source of this part can be found from Escherichia coli str. K-12 substr. MG1655, complete genome. Due to the long length of the second part, you will not sequence your plasmid completely.Source
Bglbricking of M10035, Ag43 (autotransporter and passenger)PCR Oso003 and Oso002 on E. coli MG1655 genomic DNA (1600 bp, EcoRI/BamHI)
Sub into pBca9495KC-Bca1144#5 (EcoRI/BamHI, 910+2974, L)
Product is pBca9495KC-M10035 {
Oso003 Cloning of Ag43, long ccataGAATTCatgAGATCTacgctgacgctgaacgacag
Oso002 Cloning of Ag43 GCttaggatcctcagaaggtcacattcagtg