Types | DnaRegion
|
Roles | Coding
CDS
|
Sequences | BBa_M10048_sequence (Version 1)
|
Description
References:
http://www.ebi.ac.uk/interpro/IEntry?ac=IPR000258
The part encodes for the region of the ice-nucleation protein (Inp) containing motifs of 48-amino acid repeats, responsible for seeding ice crystals. The endogenous function may have use in a surface display assay.
As an outer membrane protein, the full Inp has been previously demonstrated for use in cell-surface display:
Jung HC, Lebeault JM, Pan JG. Surface display of Zymomonas mobilis levansucrase by using the ice-nucleation protein of Pseudomonas syringae. Nature Biotechnology. 16, 576-580 (1998).
Shimazu M, Mulchandani A, Chen W. Cell surface display of organophosphorus hydrolase using ice nucleation protein. Biotechnology Progress. 17, 76-80 (2001).
Notes
None
Source
pBca1256-Bca1346