BBa_M10088

BBa_M10088 Version 1

Component

Source: http://parts.igem.org/Part:BBa_M10088

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: David de Renzy

Date created: 2009-05-05 11:00:00

Date modified: 2015-05-08 01:13:51

{˂
TshA!} | N-terminal displaying Tsh Autotransporter

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_M10088_sequence (Version 1)

Description

Autotransporters are mainly used for the translocation of large proteins or protein domains across the outer membrane. The autotransporter protein contains it own machinery, a transporter domain, to secrete its covalently attached, N-terminal passenger across the outer membrane. After secretion to the periplasm through normal SEC dependent pathways, the autotransporter portion inserts into the outer membrane as a Beta-barrel
forming oligomeric pores, similar to porins. The covalently linked passenger peptide will pass through this self-formed beta-barrel folding progressively into its native configuration as it reaches the cell surface. The passenger peptide remains in a non-native conformation while it is in the cytoplasm and the periplasm.

This part has been successfully used to display...

Notes

This part was design to attach to the C-terminal end of a desired exoprotein passengers, therefore it does not contain a start codon, but does contain a stop codon. Also, it does not include the necessary N-terminal Prepro sequences for SEC secretion across the perplasmic membrane.

Source

Pubmed nucleotide sequence AF218073 or protein AAA24698 from Escherichia Coli genome. Selected coding sequence includes amino acid 855 to 1358.

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_M10088/1

Member of these Collections

• iGEM Parts Registry