BBa_M11076

BBa_M11076 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_M11076
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Elisabeth Linton
Date created: 2009-04-15 11:00:00
Date modified: 2015-05-08 01:13:53

NrtA: nitrate/nitrite binding protein coding region



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_M11076_sequence (Version 1)

Description

NrtA is one of four polypeptides found in the high-affinity active nitrate transport system of Synechococcus sp. JA-3-3Ab. Specifically, NrtA is a periplasmic substrate-binding protein that has a specific and high binding affinity to nitrate and nitrite. The presence of NrtA is necessary for cell survival under conditions where nitrate is the primary available nitrogen source.

The presence of available nitrate is important for photosynthesis and growth. Understanding the components of the ABC nitrate/nitrite transport system and having the ability to manipulate these proteins could be of interest in many photosynthetic growth applications. Additionally, nitrate is a nutrient that is often required for removal in wastewater treatment systems. The high binding affinity of this particular protein to nitrate could be useful in nitrate removal.

Notes

There are no restriction enzymes sites within the sequence that need to be mutated for standard Biobrick assembly.

Source

Gene Object ID: 637872195, Genome: Synechococcus sp. JA-3-3Ab, DNA Coordinates: 621322..622644

U.S. Department of Energy Joint Genome Institute Gene object ID: 637872195
http://img.jgi.doe.gov/cgi-bin/pub/main.cgi?section=GeneDetail&page=geneDetail&gene_oid=637872195

Sequence Annotation Location Component / Role(s)
Start
Coding
Stop
1,3
1,1320
1321,1323
feature/start start_codon
CDS feature/cds
stop_codon feature/stop
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_M11076/1