BBa_M11078

BBa_M11078 Version 1

Component

Source: http://parts.igem.org/Part:BBa_M11078

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Forrest Purser

Date created: 2009-04-17 11:00:00

Date modified: 2015-05-08 01:13:53

OmpC + Copper-Binding Protein

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_M11078_sequence (Version 1)

Description

Membrane display OmpC protein with inserted copper-binding protein that is expressed on the surface of Escherichia coli in an extracellular loop.

Notes

note: copper-binding protein was inserted in a Pst1 restriction enzyme site ater it was filled in using DNA Polymerase. Two nucletides were inserted at the end of the copper-binding sequence to avoid throwing off the reading frame.

Source

Omp C is native to E.coli

See
Xu Z, Lee SY. (1999). Display of Polyhistidine Peptides on the Escherichia coli Cell Surface by Using Outer Membrane Protein C as an Anchoring Motif. Applied and Environmental Microbiology. 65(11): 5142-5147

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Copper binding Protein	969,1307	feature/misc, sequence_feature
Filler	1308,1308	feature/misc, sequence_feature
Filler	968,968	feature/misc, sequence_feature
start	1,3	start_codon, feature/start
stop	1512,1514	stop_codon, feature/stop

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_M11078/1

Member of these Collections

• iGEM Parts Registry