Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Angela Dixon
Date created: 2010-04-20 11:00:00
Date modified: 2015-05-08 01:13:53
pRS426 plasmid vector for S. cerevisiae
| Types | DnaRegion |
| Roles | Plasmid_Backbone plasmid_vector |
| Sequences | BBa_M11200_sequence (Version 1) |
Description
Plasmid backbone used to put BioBricks into S. cerevisiae. Contains selection for uracil negative,ampicillin and LacZ.Notes
Used mutagenesis to remove PstI at site 395???400 and XbaI at site 5294???5299. Rearrange EcoRI, XbaI, Pst1, and SpeI sites to be compatible with BioBrick standard.Source
Modified genomic sequence of pRS426 vector| Sequence Annotation | Location | Component / Role(s) |
| XbaI EcoR1 SpeI PstI Amp resistance Uracil Synthase lacZ | 2038,2043 2032,2037 2056,2061 2062,2067 3324,4182 417,1218 1589,2165 | feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature sequence_feature feature/misc feature/protein CDS sequence_feature feature/misc |