Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Andrea Olson
Date created: 2011-04-17 11:00:00
Date modified: 2015-05-08 01:13:55
Suicide Gene with GFP Tag
| Types | DnaRegion |
| Roles | engineered_region Intermediate |
| Sequences | BBa_M1206_sequence (Version 1) |
Description
Suicide Gene that utilizes T4 Holin and Lysozymes to degrade the cell wall and perforate the inner membrane of the cell, resulting in the death of the cell. A T4 antiholin is present when the cell is not to be terminated. The GFP marker is in place to ensure that kill gene is working when below 42C.The suicide gene is controlled by a tetracycline repressor.
Notes
Must consider lag time when transitioning from above 42C to below. It will take time for the repressor proteins to degrade once the coding for them stops when below 42C.Source
Kill Gene Design Ideahttp://2010.igem.org/Team:HKU-Hong_Kong
| Access | Instance | Definition |
| public public public public public public public public public public | BBa_R0040 BBa_J61102 BBa_E0040 BBa_K112805 BBa_K112806 BBa_B0015 BBa_J23116 BBa_J61101 BBa_K112807 BBa_B0010 | p(tetR) BBa_J61102 GFP BBa_K112805 BBa_K112806 BBa_B0015 BBa_J23116 BBa_J61101 BBa_K112807 BBa_B0010 |