Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Grant Robinson
Date created: 2010-05-10 11:00:00
Date modified: 2015-06-15 01:09:10
BioBrick-Compatible Broad-Host-Range Vector pPMQAK1
| Types | DnaRegion |
| Roles | Plasmid plasmid |
| Sequences | BBa_M12068_sequence (Version 1) |
Description
This shuttle vector appears to be useful for the expression of BioBricks in multiple chassis, including non-Escherichia coli microbes. According to the authors:"This vector contains the replicon of the broad-host-range plasmid RSF1010, designed for replication in different cyanobacterial, and even non-cyanobacterial, strains. The RSF1010 replicon of the IncQ group is well known for allowing plasmids to replicate in a large variety of gram negative bacteria, but there are examples of successful replication
in gram positive bacteria as well. In fact, the replicative and conjugal abilities of IncQ type plasmids make them the most wide-spread bacterial replicons known. This includes, for example, bacteria from the genus Pseudomonas, cyanobacterial genera such as Synechococcus, Prochlorococcus and Agrobacterium tumefaciens."
*Please Note: I did not design this vector, and am not an expert on either its construction or its function. All information disclosed is based on my understanding of the vector and its potential usefulness, and there is a possibility some claims have been misrepresented. That said, to the best of my knowledge, this information is accurate.*
Notes
According to the authors' article, although BioBrick expression in non-Escherichia coli organisms was indeed possible with the use of the vector, there are issues with promoter expression (in particular, Plac and Ptet are essentially dysfunctional) and apparent issues with RNA Polymerase interactions with at least some protein-mediated activation/inhibition reactions; in particular, one designed promoter, Ptrc2O was properly repressed by LacI but was unable to return to constitutive activity with the addition of surplus IPTG. In short, protein expression via the pPMQAK1 shuttle vector is apparently viable, but dedicated promoters may well have to be designed depending on the chassis.Source
http://www.ncbi.nlm.nih.gov/pubmed/20236988 [Location of the original article containing reference to pPMQAK1.]http://www.ncbi.nlm.nih.gov/nuccore/292601742 [Location of the pPMQAK1 vector expressed here in GenBank.]