BBa_M1524

BBa_M1524 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_M1524
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sarah Allred
Date created: 2012-04-09 11:00:00
Date modified: 2015-05-08 01:13:56

Thermal Hysteresis (Antifreeze) Protein (variant YL1) with 8-His tag



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_M1524_sequence (Version 1)

Description

This is a thermal hysteresis (antifreeze) protein. It has up to 100 times the specific activity of fish antifreeze proteins. There are four variants of the protein (YL1-4). This is variant YL1.

Notes

The codons were optimized to be used in E. coli, and any restriction enzyme sites were removed.

Source

This part comes from Tenebrio molitor. Amino acid sequences for four variants are found in a paper in Nature entitled, "Hyperactive antifreeze protein from beetles" (Graham et al., 1997).

Sequence Annotation Location Component / Role(s)
cds
1,477
feature/cds CDS
igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_M1524/1