BBa_M1525

BBa_M1525 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_M1525
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sarah Allred
Date created: 2012-04-09 11:00:00
Date modified: 2015-05-08 01:13:56

Thermal Hysteresis (Antifreeze) Protein (variant YL4) with 8-His tag



    • Details

    Types
    DnaRegion

    Roles
    CDS

    Coding

    Sequences BBa_M1525_sequence (Version 1)

    Description

    This is a thermal hysteresis (antifreeze) protein. It has up to 100 times the specific activity of fish antifreeze proteins. There are four variants of the protein (YL1-4). This is variant YL4.

    Notes

    The codons were optimized to be used in E. coli, and any restriction enzyme sites were removed.

    Source

    This part comes from Tenebrio molitor. Amino acid sequences for four variants are found in a paper in Nature entitled, "Hyperactive antifreeze protein from beetles" (Graham et al., 1997).

    Sequence Annotation Location Component / Role(s)
    cds
    		1,477
    		feature/cds CDS

    igem#experience
    None
     
    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_M1525/1
     

    Attachments

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