BBa_M1525

BBa_M1525 Version 1

Component

Source: http://parts.igem.org/Part:BBa_M1525

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Sarah Allred

Date created: 2012-04-09 11:00:00

Date modified: 2015-05-08 01:13:56

Thermal Hysteresis (Antifreeze) Protein (variant YL4) with 8-His tag

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_M1525_sequence (Version 1)

Description

This is a thermal hysteresis (antifreeze) protein. It has up to 100 times the specific activity of fish antifreeze proteins. There are four variants of the protein (YL1-4). This is variant YL4.

Notes

The codons were optimized to be used in E. coli, and any restriction enzyme sites were removed.

Source

This part comes from Tenebrio molitor. Amino acid sequences for four variants are found in a paper in Nature entitled, "Hyperactive antifreeze protein from beetles" (Graham et al., 1997).

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
cds	1,477	feature/cds, CDS

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_M1525/1

Member of these Collections

• iGEM Parts Registry