BBa_M31022

BBa_M31022 Version 1

Component

Source: http://parts.igem.org/Part:BBa_M31022

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: bryan hernandez

Date created: 2007-02-28 12:00:00

Date modified: 2015-05-08 01:13:59

Gene II (RBS X.1 silent mutant)

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_M31022_sequence (Version 1)

Description

preserves AA sequence of gene II while weakening the internal RBS X which will be annotated as RBS X.1.

Notes

I wanted to weaken RBS X and preserve II AA sequence.

II: gca ttt gag ggg gat tca
X RBS: gca ttt gag ggg gat tca
AA seq: ALA PHE GLU GLY ASP SER
-10^
X.1 RBS region: gcg ttc gaa ggc gac tcg
New AA seq: ALA PHE GLU GLY ASP SER

Source

gene II

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
RBS X.1	882,897	point_mutation, feature/s_mutation
SpeI	1234,1239	feature/misc, sequence_feature

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_M31022/1

Member of these Collections

• iGEM Parts Registry