BBa_M31500

BBa_M31500 Version 1

Component

This part has been discontinued.

Source:
http://parts.igem.org/Part:BBa_M31500
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Tiffany Guo
Date created: 2007-02-27 12:00:00
Date modified: 2015-05-08 01:14:00

refactored M13K07 phage fragment



    • Details

    Types
    DnaRegion

    Roles
    engineered_region

    Intermediate

    Sequences BBa_M31500_sequence (Version 1)

    Description

    M13K07 fragment from bp 1-2222 (HpaI site to BamHI site), refactored 1) to eliminate overlaping regions between promoters, ribosome binding sites, open reading frames, and 2) to add restriction enzyme sites for easy separation of different parts of the genetic sequence.

    Notes

    Had to make sure 1) overlapping promoter/rbs/orf sequences were not repeated, 2) restriction enzyme sites were unique and did not cut through important parts.

    Source

    M13K07 fragment from bp 1-2222 (HpaI site to BamHI site)

    igem#experience
    None
     
    igem#sampleStatus
    Discontinued
    igem#status
    Deleted
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_M31500/1
     

    Attachments

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