BBa_M31520

BBa_M31520 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_M31520
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Han Zhu
Date created: 2007-02-27 12:00:00
Date modified: 2015-05-08 01:14:00

g8 modified to be independent of g3 promoter



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_M31520_sequence (Version 1)

Description

This is a modified g8 that has point mutations to delete the promoter of g3 inside it. It also has an EcoRI restriction site added to the end. Therefore, it operates independent of g3.

Notes

All point mutations made were checked to make sure they really are silent mutations that will not change the amino acid sequence of p8.

Source

atgaaaaagtctttagtcctcaaagcctctgtagccgttgctaccctcgttccgatgctgtctttcgctgctgagggtgacgatcccgcaaaagcggcctttaactccctgcaagcctcagcgaccgaatatatcggttatgcgtgggcgatggttgttgtcattgtcggcgcaactatcggtatcaagctgtttaagaaattTacTtcCaaGgcGagTtgaGAATTC

Sequence Annotation Location Component / Role(s)
g8 (modified)
EcoRI
BglII
7,228
229,234
1,6
feature/protein CDS
feature/misc sequence_feature
feature/misc sequence_feature
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_M31520/1