BBa_M31520

BBa_M31520 Version 1

Component

Source: http://parts.igem.org/Part:BBa_M31520

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Han Zhu

Date created: 2007-02-27 12:00:00

Date modified: 2015-05-08 01:14:00

g8 modified to be independent of g3 promoter

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_M31520_sequence (Version 1)

Description

This is a modified g8 that has point mutations to delete the promoter of g3 inside it. It also has an EcoRI restriction site added to the end. Therefore, it operates independent of g3.

Notes

All point mutations made were checked to make sure they really are silent mutations that will not change the amino acid sequence of p8.

Source

atgaaaaagtctttagtcctcaaagcctctgtagccgttgctaccctcgttccgatgctgtctttcgctgctgagggtgacgatcccgcaaaagcggcctttaactccctgcaagcctcagcgaccgaatatatcggttatgcgtgggcgatggttgttgtcattgtcggcgcaactatcggtatcaagctgtttaagaaattTacTtcCaaGgcGagTtgaGAATTC

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
g8 (modified)	7,228	feature/protein, CDS
EcoRI	229,234	feature/misc, sequence_feature
BglII	1,6	feature/misc, sequence_feature

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_M31520/1

Member of these Collections

• iGEM Parts Registry