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BBa_M31784

BBa_M31784 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_M31784

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Emilienne Repak

Date created: 2007-02-28 12:00:00

Date modified: 2015-05-08 01:14:00

Gene VIII w/o Gene III Promoter + w/ mCherry

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Details

Types	DnaRegion
Roles	CDS Coding
Sequences	BBa_M31784_sequence (Version 1)

Description

This produces gene VIII (without the Gene III promoter) with mCherry fused to it.

Notes

I had to remove the Gene III Promoter. Then I had to try to add the mCherry onto the extracellular side of protein VIII, which I believe is the N-terminus, which I think is coded on the 5' side of the gene, so I added mCherry before Gene VIII.

Source

M13K07 and the Standard Registry of Parts, thanks to work done in Roger Tsien's laboratory at UCSD.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
mCherry gene VIII ex-Gene X RBS	1,714 715,936 914,936	feature/misc sequence_feature sequence_feature feature/misc sequence_feature feature/misc