Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Scott Runyon
Date created: 2010-06-01 11:00:00
Date modified: 2015-05-08 01:14:01
NarL: Regulates nitrate reduction (strong RBS)
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_M33030_sequence (Version 1) |
Description
The two Ecoli K-12 cell membrane proteins, NarX and NarQ, are responsive to nitrate and nitrate in the environment. When nitrate levels increase, When NarX (a membrane protein) binds nitrate, it autophosphorylates and then acts as a histidine kinase on the NarL protein. When NarL is phosphorylated, it binds bacterial DNA and acts as a repressor/activator, depending on the particular operon.they transfer a signal to the NarL gene. The NarL protein is a cytoplasmic transcription factor that serves as the second component in the two-component Nar pathway. When NarL is phosphorylated, it binds bacterial DNA and acts as a repressor/activator, depending on the particular operon.With increased nitrate, NarL upregulates the expression of three nitrate reducing enzymes, nitrate reductase (narGHJI), dimethyl sulfoxide/trimethylamine-N-oxide reductase (dmsABC), and fumarate reductase (frdABCD). Therefore, expression of NarL appears to be directly correlated to the presence of nitrate in the system, and could potentially be used with Bba_216005, a nitrate sensitive promotor, and a color generator in a novel nitrate sensing construct. For this part, we have added BBa_J23106, a medium strength constitutive promoter, and moderate strength RBS Bba_B0030.
Notes
Initial NarL part was ordered with BBa sites but without promoter or RBS. Primers were ordered separately that added these and of course a new set of identical restriction sites, the original ones removed.Source
Coding sequence taken from NCBI: http://www.ncbi.nlm.nih.gov/gene/945795and synthesized in Palo Alto by DNA 2.0