Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Christopher Brunson
Date created: 2010-06-04 11:00:00
Date modified: 2015-05-08 01:14:01
CDS+RBS for Inorganic mercury-sensitive regulatory protein merR coding sequence with RBS
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_M33334_sequence (Version 1) |
Description
This contains the coding sequence for merR (mercury repressor protein) with an added medium strength RBS. It is part of the mer operon from Thermus thermophilus HB27, and binds to the mer promoter in the absence of inorganic mercury. if inorganic mercury is present, it binds to merR, which undergoes a conformational change that allows for transcription of the downstream operon.Notes
A medium strength RBS had to be added (AAAGAGGAGAAA)Source
Thermus thermophilus HB27, complete genomeGenBank: AE017221.1
773178..773606
| Sequence Annotation | Location | Component / Role(s) |
| ArsR binding site -35 sequence -10 sequence rbs arsR BBa_J33201 | 36,54 57,62 80,85 108,112 119,472 1,518 | operator feature/operator feature/promoter promoter promoter feature/promoter ribosome_entry_site feature/rbs feature/cds CDS feature/BioBrick engineered_region |