Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Divya Arcot
Date created: 2011-05-08 11:00:00
Date modified: 2015-05-08 01:14:02
Exported Yeast TIMP-1
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_M34543_sequence (Version 1) |
Description
TIMP-1 gene under control of a promoter with an export signal sequence and a terminator sequence. This part causes a yeast cell to secrete the human TIMP-1 gene to inhibit matrix metalloproteinases (MMPs).Notes
We wanted to use a signal sequence which exported the greatest amount of the TIMP-1 protein, so we chose the alpha F sequence over others. We also chose to use yeast as a chassis because TIMP-1 is a human gene and yeast would be able to glycosylate it. However, yeast glycosylate the protein in a manner that is slightly different than in humans.Source
The human TIMP-1 gene is located on the X chromosome.For the terminator sequence: pOBD2.
Promoter is from the Parts Registry (Part BBa_I766556)
Signal Sequence Source: http://peds.oxfordjournals.org/content/11/2/75.full.pdf (use alpha F sequence)
| Sequence Annotation | Location | Component / Role(s) |
| Secretion Signal Sequence pAdh (Strong) Promoter TIMP-1 Gene Terminator Sequence | 2434,2689 1,1501 1502,2433 2690,2878 | sequence_feature feature/dna feature/promoter promoter feature/dna sequence_feature feature/dna sequence_feature |