BBa_M36024

BBa_M36024 Version 1

Component

Source: http://parts.igem.org/Part:BBa_M36024

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jon Rodriguez

Date created: 2011-05-02 11:00:00

Date modified: 2015-05-08 01:14:02

phiX174 gene A - mutated RBS's and start codons of B, K and C.

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_M36024_sequence (Version 1)

Description

Took the wt gene A from coliphage phiX174, and then mutated it synonymously in order to destroy the RBS's and start codons of the overlapping genes B, K, and C.

Notes

Made only synonymous changes, and tried to keep the codon usage bias as similar as possible to wt.

Source

wt phiX174, then mutated by Jon Rodriguez and Adrian Sierra

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Start Codon	1,3	feature/start, start_codon
Stop Codon	1540,1542	feature/stop, stop_codon
CDS	1,1542	feature/cds, CDS
a->t	1086,1086	point_mutation, feature/s_mutation
t->c	1089,1089	point_mutation, feature/s_mutation
a->g	1095,1095	feature/s_mutation, point_mutation
g->t	1446,1446	feature/s_mutation, point_mutation
t->c	1449,1449	feature/s_mutation, point_mutation
t->c	1458,1458	point_mutation, feature/s_mutation
t->c	1527,1527	point_mutation, feature/s_mutation
t->c	1530,1530	point_mutation, feature/s_mutation
a->g	1539,1539	point_mutation, feature/s_mutation

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• rdf-schema#seeAlso: P03631
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_M36024/1

Member of these Collections

• iGEM Parts Registry