| Types | DnaRegion
|
| Roles | Reporter
engineered_region
|
| Sequences | BBa_M36039_sequence (Version 1)
|
Description
In order to measure the bilirubin content in urine, we will design an actuator that that communicates the presence of bilirubin. Our product, a biological actuator, is the protein encoded by UnaG that will be continually transcribed and translated within the E.coli cells and then exported from them so that it can interact with any bilirubin present. An important distinction is that the gene is not activated by the presence of bilirubin???the protein will be produced regardless of the concentration of bilirubin. The UnaG protein will only be detectable, by measuring its fluorescence, when it interacts chemically with the bilirubin molecule.
Notes
We need a secretion tag that would export our protein outside of the outer-most cell membrane such that it could interact with bilirubin in solution without the bilirubin being imported into the cell.
Source
NCBI Gene Bank contained the sequence of the protein originally found in Anguilla japonica.
