BBa_M36093

BBa_M36093 Version 1

Component

Source: http://parts.igem.org/Part:BBa_M36093

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Joseph Shih

Date created: 2013-05-07 11:00:00

Date modified: 2015-05-08 01:14:03

S. cerevisiae sensor test rig

Details

• Types: DnaRegion
• Roles: plasmid_vector; Plasmid_Backbone
• Sequences: BBa_M36093_sequence (Version 1)

Description

S. cerevisae test vector for sensors. Has a DasherGFP protein coding sequence which produces a GFP protein. Comes with high copy number origin, kanamycin resistance, and leucine yeast selection; can replace with low copy number origin if ordering from DNA 2.0

Notes

None

Source

Vector is based off of pJ1201-03c plasmid developed by DNA2.0

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Pamp	1,128	feature/promoter, promoter
KanR	129,938	feature/cds, CDS
pUCori	939,1742	feature/misc, sequence_feature
student sensors go here	1757,2171	feature/misc, sequence_feature
DasherGFP	2178,2883	CDS, feature/cds
CYC1 polyA	2885,3136	polyA_site, feature/polya
Pleu	3155,3654	promoter, feature/promoter
Leu	3655,4749	feature/cds, CDS
Leu polyA	4750,4849	feature/polya, polyA_site
2 um yeast ori	4854,6299	feature/misc, sequence_feature
yeast Kozak	2172,2177	feature/rbs, ribosome_entry_site

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_M36093/1

Member of these Collections

• //plasmidbackbone
• iGEM Parts Registry
• igem_plasmid_vectors