BBa_M36097

BBa_M36097 Version 1

Component

Source: http://parts.igem.org/Part:BBa_M36097

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Joseph Shih

Date created: 2013-04-22 11:00:00

Date modified: 2015-05-08 01:14:03

Mammalian sensor test rig

Details

• Types: DnaRegion
• Roles: plasmid_vector; Plasmid_Backbone
• Sequences: BBa_M36097_sequence (Version 1)

Description

Mammalian test vector for sensors. Has a Comet protein coding sequence which produces a GFP-like protein. Comes with high copy number origin and ampicillin resistance; can replace with low copy number origin and kanamycin resistance if ordering from DNA 2.0.

Notes

Comet is a protein developed by DNA2.0

Source

All of the parts were designed by BIOE44 instructors in conjunction with DNA 2.0.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
rpo_bla transcription terminator	1,425	stop_codon, feature/stop
student sensors go here	507,576	sequence_feature, feature/misc
kozak	587,593	ribosome_entry_site, feature/rbs
Comet	594,1307	feature/cds, CDS
BGH polyA	1314,1439	polyA_site, feature/polya
SV40 promoter	1551,1894	promoter, feature/promoter
puromycinR	2005,2604	feature/cds, CDS
SV40 polyA	2732,2862	feature/polya, polyA_site
AmpR	2908,3768	feature/cds, CDS
Pamp	3769,3946	feature/promoter, promoter
pUCori	3947,4751	sequence_feature, feature/misc

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_M36097/1

Member of these Collections

• //plasmidbackbone
• iGEM Parts Registry
• igem_plasmid_vectors