Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Adrian Sierra
Date created: 2011-05-02 11:00:00
Date modified: 2015-05-08 01:14:03
Lysis gene E for phiX174, synonymous mutation on gene J's rbs inside E
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_M36111_sequence (Version 1) |
Description
Gene E is the lysis gene for phiX174. Inside this gene is the Shine-Dalgarno sequence for the subsequent gene J. In order to facilitate decompression of the genome, the Shine-Dalgarno sequence of J was synonymously mutated at index 270 and 273 of the E gene, g->a and g->a.Notes
When mutating base pairs, first priority was mutating into a c or t, second was maintaining a similar codon usage as the original.Source
wild type phiX174 gene E.| Sequence Annotation | Location | Component / Role(s) |
| start codon stop codon g->a g->a CDS | 1,3 274,276 270,270 273,273 1,276 | feature/start start_codon feature/stop stop_codon point_mutation feature/s_mutation point_mutation feature/s_mutation CDS feature/cds |