| Types | DnaRegion
|
| Roles | polypeptide_domain
Protein_Domain
|
| Sequences | BBa_M36148_sequence (Version 1)
|
Description
This part encodes the gene AlgA, or alginic acid subunit A. AlgA plays a role in the biosynthesis of alginate, a multi-use substance with thickening properties during two major steps of the process. AlgA encodes the enzyme phosphomannose isomerase???GDP-mannose pyrophosphorylase, which is necessary to convert fructose-6-phosphate to mannose-6-phosphate, and also mannose-1-phosphate to GDP-mannose (Rehm et al. 2009). When paired with other parts used in the alginate synthesis process, AlgA can successfully create algin. This part was optimized for tranformation of Saccharomyces cerevisiae using the pD1201 plasmid from DNA 2.0.
Notes
Considerations for the sequence design included eliminating repeats of over 10 base pairs, and eliminating restriction sites for BsaI, BbsI and BsmBI. We optimized the codons for use in yeast, Saccharomyces cerevisiae. We also included a FLAG tag to help with the detection of the sequence during Western blot tests.
Source
The part comes from the sequence of algA isolated in bacteria Pseudomonas aeruginosa. More information about the gene, including the original sequence, can be found here: http://www.ncbi.nlm.nih.gov/gene/879142. The part also contains a sequence from Gene Designer encoding a FLAG tag for detection in Western blots.
