BBa_M36289

BBa_M36289 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_M36289
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Victoria Robles, Kyung Ri Park, Ken Xiong
Date created: 2011-12-09 12:00:00
Date modified: 2015-05-08 01:14:04

medium 5' Bicistronic UTR (BD8)



Types
DnaRegion

Roles
RBS

ribosome_entry_site

Sequences BBa_M36289_sequence (Version 1)

Description

Much like BD21 (BBa_M36289), this part is a 5' UTR of medium strength (varying with it upstream promoter) based on bicistronic junction architecture. The first RBS is strong and drives expression of short leader cistron. The second RBS is internal to the first cistron and controls translation of gene of interest. Architecture is optimized so that downstream CDS for gene of interest does not have a chance to form a secondary structure with the 5' UTR. See BIOFAB.org for more information.

Notes

-

Source

BIOFAB.org
apFAB567

Sequence Annotation Location Component / Role(s)
dna
1,88
sequence_feature feature/dna
igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_M36289/1