BBa_M36534

BBa_M36534 Version 1

Component

Source: http://parts.igem.org/Part:BBa_M36534

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Inderpreet Kaur Hayer

Date created: 2015-10-23 11:00:00

Date modified: 2015-12-09 02:45:04

Arsenate Bioremediation Actuator Plasmid Containing ACR 1 and ACR 2 Genes

Details

• Types: DnaRegion
• Roles: Plasmid; plasmid
• Sequences: BBa_M36534_sequence (Version 1)

Description

This part was made in order to enhance arsenic absorption and resistance in S. cerevisae.

DNA 2.0 Plasmid (pD1204) with GAL1 inducible promoter, high copy number, and Uracil as the selectable marker
Part contains (in order):
RBS: consensus yeast Kozak Sequence
Spacer sequence
ACR2 gene
Stop codon
Spacer sequence
RBS: consensus yeast Kozak Sequence
Spacer sequence
ACR1 gene
Stop codon

Notes

ACR 2 was ordered first due its increased importance vs. ACR 1.

Source

DNA 2.0 for general plasmid design including restriction sites, GAL1 inducible promoter, high copy number, and
Sequence for ACR 1 and 2 from yeastgenome website.
http://www.yeastgenome.org/locus/S000006403/sequence
http://www.yeastgenome.org/locus/S000006404/sequence

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_M36534/1

Member of these Collections

• iGEM Parts Registry