BBa_M36595

BBa_M36595 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_M36595
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jessica Lam
Date created: 2013-06-09 11:00:00
Date modified: 2015-05-08 01:14:05

Bicistronic design RBS



Types
DnaRegion

Roles
ribosome_entry_site

RBS

Sequences BBa_M36595_sequence (Version 1)

Description

This part is a bicistronic design ribosome binding site used in conjunction with BBa_M36596

Notes

Considerations of the benefits and convenience of having a bicsistronic design led us to choose this option, in spite of the fact that the bicistronic design is targeted for E. Coli and has not yet been tested in S. Elongatus. Interestingly enough, although the part was originally designed for expression of the hydrogenase protein in cyanobacteria, it has so far only been proved for the expression of hydrogenase protein in E. Coli.

Source

Bicistronic RBS:
BioFAB ID: pFAB1655
Mutalik VK, Guimaraes JC, Cambray G, Mai QA, Christoffersen MJ, Martin L, Yua A, Lam C, Rodriguez C, Bennett G, et al. Quantitative estimation of activity and quality for collections of functional genetic elements. Nat. Methods. 2013;10:347-353. BioFAB. (http://io.biofab.org:5000/simple/randomized_bds)

igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_M36595/1