Types | DnaRegion
|
Roles | ribosome_entry_site
RBS
|
Sequences | BBa_M36595_sequence (Version 1)
|
Description
This part is a bicistronic design ribosome binding site used in conjunction with BBa_M36596
Notes
Considerations of the benefits and convenience of having a bicsistronic design led us to choose this option, in spite of the fact that the bicistronic design is targeted for E. Coli and has not yet been tested in S. Elongatus. Interestingly enough, although the part was originally designed for expression of the hydrogenase protein in cyanobacteria, it has so far only been proved for the expression of hydrogenase protein in E. Coli.
Source
Bicistronic RBS:
BioFAB ID: pFAB1655
Mutalik VK, Guimaraes JC, Cambray G, Mai QA, Christoffersen MJ, Martin L, Yua A, Lam C, Rodriguez C, Bennett G, et al. Quantitative estimation of activity and quality for collections of functional genetic elements. Nat. Methods. 2013;10:347-353. BioFAB. (http://io.biofab.org:5000/simple/randomized_bds)