Types | DnaRegion
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Roles | Reporter
engineered_region
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Sequences | BBa_M36629_sequence (Version 1)
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Description
Encodes the FLAG epitope, which has the amino acid sequence of DYKDDDDK. Can be added to either the N- or C- terminus of a protein. No start codon included.
Notes
1. Avoid BsaI, BbsI, and BsmBI restriction sites
2. Avoid repeats over 10bp in length.
Source
The DNA sequence was generated from the amino acid sequence (DYKDDDDK) using IDT Codon Optimizer for E. coli K12. It was then modified to remove certain restriction sites and repeats.