Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Gordon Sun
Date created: 2014-05-05 11:00:00
Date modified: 2015-05-08 01:14:05
Hypoxia Sensor
| Types | DnaRegion |
| Roles | engineered_region Measurement |
Description
Part utilizes FNR transcription factor with E. Coli constitutive promoter, which regulates pepT promoter downstream with Gemini actuator (with only LacZ gene).FNR regulated by O2, inactive when exposed to O2; requires Glutathione and cysteine desulfurase to reactivate, of which cysteine desulfurase is already present within E. Coli.
Notes
Modification GAG to GAA at position 418 and ACG to ACA at 421 to remove a restriction site but preserve the amino acid sequence.Source
1. Unden, Gottfried, and Jan Schirawski. "The Oxygen-responsive Transcriptional Regulator FNR of Escherichia Coli: The Search for Signals and Reactions."Molecular Microbiology 25.02 (1997): 205-10. Web.2. ???Part:BBa_J23100.??? Registry of Standard Biological Parts. Web.
3. ???Part:BBa_B0015.??? Registry of Standard Biological Parts. Web.
4. Lombardo, Mary-Jane, Angela A. Lee, Tina M. Knox, and Charles G. Miller. "Regulation of the Salmonella Typhimurium PepT Gene by Cyclic AMP Receptor Protein (CRP) and FNR Acting at a Hybrid CRP-FNR Site."Journal Of Bacteriology 179.6 (1997): 1909-917. American Society for Microbiology. Web. 04 May 2014.