Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nathan Barnett, Meghan Bowler, Kian Torabian
Date created: 2011-05-16 11:00:00
Date modified: 2015-05-08 01:14:07
Type II Secreted Antifreeze Actuator in E. coli (1-Repeat) Correct
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_M36982_sequence (Version 1) |
Description
5'UTR, signal peptide, non-glycolated antifreeze protein, and terminator that will as whole transcribe and secrete antifreeze protein outside the cell membrane.Notes
This composite part consists of a strong 5' UTR Sequence, followed by a signal peptide for secretion from E.coli, the non-glycolated AFP 1-mer, and finally a strong termination sequence. The 5' UTR and termination sequences are very strong because we want high transcription and only transcription of the region we coded before the termination sequence. The signal peptide is N-terminal and is native to E.coli. This composite is a shortened version of the 5-repeat Type II Secreted Antifreeze Actuator (BBa_M36990).Source
Pseudopleuronecta americanus (White Flounder) and E. Coli| Access | Instance | Definition |
| public public public public | BBa_M36009 BBa_M36981 BBa_M36997 BBa_M36010 | BBa_M36009 BBa_M36981 BBa_M36997 BBa_M36010 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_M36009 BBa_M36981 BBa_M36997 BBa_M36010 | 1,47 48,119 120,221 222,303 | BBa_M36009 BBa_M36981 BBa_M36997 BBa_M36010 |