Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nathan Barnett, Meghan Bowler, Kian Torabian
Date created: 2011-04-27 11:00:00
Date modified: 2015-05-08 01:14:07
Type II Secreted Antifreeze Actuator in E. coli (1-Repeat)
| Types | DnaRegion |
| Roles | Project engineered_region |
| Sequences | BBa_M36994_sequence (Version 1) |
Description
5'UTR, signal peptide, non-glycolated antifreeze protein, and terminator that will as whole transcribe and secrete antifreeze protein outside the cell membrane.Notes
This design has a "ANAAAAAALTA" sequence that is only roughly used once, unlike other AFP sequences which have more repeats of this sequence.Source
Based on a protein sequence from the white flounder, Pseudopleuronecta americanus.| Access | Instance | Definition |
| public public public public | BBa_M36009 BBa_M36726 BBa_M36997 BBa_M36010 | BBa_M36009 BBa_M36726 BBa_M36997 BBa_M36010 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_M36009 BBa_M36726 BBa_M36997 BBa_M36010 | 1,47 56,121 130,231 240,321 | BBa_M36009 BBa_M36726 BBa_M36997 BBa_M36010 |