Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Zach Banks
Date created: 2012-05-14 11:00:00
Date modified: 2015-05-08 01:14:07
flipable pCat promoter regulated by fimE recombinase
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_M39413_sequence (Version 1) |
Description
pCat promoter ([[BBa_I14033]]) flanked by regions ([[BBa_K137010]], [[BBa_K137008]]) recognized by fimE recombinase ([[BBa_K137007]]).pCat is a constitutive promoter, which enables this device to be used as a switch to constitutively express two different regions. By default, the upstream sense strand is promoted. When flipped, the downstream antisense strand is promoted.
Additionally, downstream of the promoter is a terminating sequence [[BBa_B0015]]. This sequence is to prevent "backwards" transcription before the promoter is flipped. It is between the recombinant regions, causing it to flip with the promoter.
pCat can be replaced with another promoter for additional regulation if needed.
Notes
The double terminator placed before the promoter prevents any transient backwards transcription. Although the amount of transcription would be small, this design allows for stronger "digital" behavior.Source
This part is a combination of several other parts from the registry| Access | Instance | Definition |
| public public public public | BBa_K137010 BBa_B0015 BBa_I14033 BBa_K137008 | fimE IRL BBa_B0015 Cat IRR |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K137010 BBa_B0015 BBa_I14033 BBa_K137008 | 1,35 44,172 181,218 227,261 | fimE IRL BBa_B0015 Cat IRR |