Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: han zhang
Date created: 2015-04-09 11:00:00
Date modified: 2015-05-08 01:14:09
pCstA+RBS+amilGFP used to indication of low glucose concentration
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_M45698_sequence (Version 1) |
Description
This BioBrick comprises a ligation of the registry parts for the promoter PcstA (BBa_K118011) and the RBS (BBa_B0034).The PcstA promoter is cAMP activated. Under low glucose concentrations, there is increased activity by adenylate cyclase. This results in cAMP binding to the cAMP receptor protein, and activating the promoter for downstream expression (more information on this part can be found on its registry page here).
This promoter has been ligated to an RBS as an intermediate step of ligations. By adding a coding region followed by terminator after this part, one would create a glucose sensitive functional unit.
This chromoprotein from the coral Acropora millepora, amilGFP, naturally exhibits strong yellow color when expressed. The color is readily visible to naked eye both in LB-culture and on agar plates. Color development can be seen in less than 24 hours of incubation.