Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Chad Lawrence Nielsen
Date created: 2015-04-09 11:00:00
Date modified: 2015-05-08 01:14:10
Botulism Toxin Type C Detector
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_M45902_sequence (Version 1) |
Description
This part is designed to detect the presence of botulism toxin type C. It is composed of a ribosome binding site, a fluorescent protein (SBFP2), a cleavage site that the toxin attacks (Syntaxin-1A), and a 10x his-tag. Ideally, this should be able to be purified after production using a nickel or copper column, eluted, and then mixed with the sample. If the toxin is in solution, it will cleave the Syntaxin-1A sequence, separating the fluorescent protein from the his-tag. This means that if the solution is run through a column again, the fluorescent protein should come out during the flow throughs rather than the elutions.Notes
Syntaxin-1A is from humans, eukaryotic organisms, so it may not work with E. coli. A blue fluorescent protein was chosen because this part is part of a set for a class project and will be different from GFP and RFP that will be released by different strains of botulism toxin.Source
Composite part created from existing parts.| Access | Instance | Definition |
| public public public public | BBa_B0035 BBa_K156010 BBa_M45901 BBa_K844000 | BBa_B0035 SBFP2 BBa_M45901 BBa_K844000 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_B0035 BBa_K156010 BBa_M45901 BBa_K844000 | 1,14 21,740 747,1610 1619,1654 | BBa_B0035 SBFP2 BBa_M45901 BBa_K844000 |