Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Joshua Charles Woodard
Date created: 2015-05-10 11:00:00
Date modified: 2015-05-11 06:29:49
Polystyrene Decomposer
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_M47007_sequence (Version 1) |
Description
This decomposer device is a combination of four genes that individually encode for esterase, alcohol dehydrogenase, alkane hydroxylase, and BVMO. When the gene products are excreted in conjunction with one another, these enzymes break down polystyrene into smaller products.Notes
This device design is untested and barrows genes from a wide variety of bacteria to function. The hope is that any general chassis will be able to run these genes and produce these products effectively, but there are a lot of unknowns between the concept in question and the actual final product. Our inspiration for the enzymatic combination above comes from these sources:http://www.sciencedirect.com/science/article/pii/S0922338X97820132
http://link.springer.com/article/10.1007/s00253-006-0443-1/fulltext.html#Fig2
Source
The esterase gene comes from a publication by Henne, Schmitz et al. from the University of Goettingen. http://www.ncbi.nlm.nih.gov/nuccore/AF223648.1The alcohol dehydrogenase gene comes from Arthrobacter; the gene sequence was decoded by J.E. Koenig of Dalhousie University
http://www.ncbi.nlm.nih.gov/nuccore/FM995514.1
The alkane hydroxylase gene is derived from Alcanivorax borkumensis by A. Hara of Kamaishi Laboratories.
http://www.ncbi.nlm.nih.gov/nuccore/AB110225.1
The BVMO gene is taken from Pseudomonas fluorescens by U.T. Bornscheuer of Greifswald University.
http://www.ncbi.nlm.nih.gov/nuccore/226880370