BBa_M50009

BBa_M50009 Version 1

Component

Source: http://parts.igem.org/Part:BBa_M50009

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Amanda Urke

Date created: 2016-11-29 12:00:00

Date modified: 2016-12-11 04:21:38

mRuby3 fluorescent protein

Details

• Types: DnaRegion
• Roles: polypeptide_domain; Protein_Domain
• Sequences: BBa_M50009_sequence (Version 1)

Description

mRuby3 is a brighter and more photostable variant of a red fluorescent protein developed by Bryce T. Bajar et al. According to their February 2016 study, mRuby3 has a maximum excitation wavelength of 558 nm, and a maximum emission wavelength of 592 nm. It can be used in FRET analysis as an acceptor fluorophore when paired with a GFP such as cometGFP.

Notes

choosing to put it in our custom part of the plasmid rather than GFP as we had originally intended

Source

http://www.nature.com/articles/srep20889

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Deletion of stop codon to integrate subsequent parts of construct	711,711	feature/stop, stop_codon

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_M50009/1

Member of these Collections

• iGEM Parts Registry